HKR1 encodes a cell surface protein that regulates both cell wall β-glucan synthesis and budding pattern in the yeast Saccharomyces cerevisiae

Tomio Yabe, Toshiko Yamada-Okabe, Shin Kasahara, Yasuhiro Furuichi, Tasuku Nakajima, Eiji Ichishima, Mikio Arisawa, Hisafumi Yamada-Okabe

    研究成果: Article査読

    34 被引用数 (Scopus)

    抄録

    We previously isolated the Saccharomyces cerevisiae HKR1 gene that confers on S. cerevisiae cells resistance to HM-1 killer toxin secreted by Hansenula mrakii (S. Kasahara, H. Yamada, T. Mio, Y. Shiratori, C. Miyamoto, T. Yabe, T. Nakajima, E. Ichishima, and Y. Furuichi, J. Bacteriol. 176:1488-1499, 1994). HKR1 encodes a type 1 membrane protein that contains a calcium-binding consensus sequence (EF hand motif) in the cytoplasmic domain. Although the null mutation of HKR1 is lethal, disruption of the 3′ part of the coding region, which would result in deletion of the cytoplasmic domain of Hkr1p, did not affect the viability of yeast cells. This partial disruption of HKR1 significantly reduced β-1,3-glucan synthase activity and the amount of β-1,3-glucan in the cell wall and altered the axial budding pattern of haploid cells. Neither chitin synthase activity nor chitin content was significantly affected in the cells harboring the partially disrupted HKR1 allele. Immunofluorescence microscopy with an antibody raised against Hkr1p expressed in Escherichia coli revealed that Hkr1p was predominantly localized on the cell surface. The cell surface localization of Hkrlp required the N-terminal signal sequence because the C-terminal half of Hkr1p was detected uniformly in the cells. These results demonstrate that HKR1 encodes a cell surface protein that regulates both cell wall β-glucan synthesis and budding pattern and suggest that bud site assembly is somehow related to β-glucan synthesis in S. cerevisiae.

    本文言語English
    ページ(範囲)477-483
    ページ数7
    ジャーナルJournal of bacteriology
    178
    2
    DOI
    出版ステータスPublished - 1996 1

    ASJC Scopus subject areas

    • 微生物学
    • 分子生物学

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