High-speed CCD imaging system for monitoring neural activity in vivo and in vitro, using a voltage-sensitive dye

Ichiro Takashima, Michinori Ichikawa, Toshio Iijima

    研究成果: Article査読

    43 被引用数 (Scopus)

    抄録

    We have designed and constructed a high-speed CCD imaging system for optically detecting neural activity from preparations stained externally with a voltage-sensitive dye, and have used this system to image evoked and epileptiform neural activity in the rat somatosensory cortex. The imaging system uses a commercially available 1/3-in. CCD chip, and it can continuously capture images for more than 8 s, at 1000 frames/s, with a spatial resolution of 128 x 62 pixels. The spatial/temporal resolution of the CCD sensor is variable by changing the geometry of on-chip binning pixels, which can be controlled by a PC/AT computer. Dye bleaching correction was not necessary for long-term imaging of epileptiform neural events, since the sensitivity of the CCD sensor was increased by combining the signal from adjacent pixels.

    本文言語English
    ページ(範囲)147-159
    ページ数13
    ジャーナルJournal of Neuroscience Methods
    91
    1-2
    DOI
    出版ステータスPublished - 1999 9月 15

    ASJC Scopus subject areas

    • 神経科学(全般)

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