High-sensitivity HPLC quantification of nonfluorescent but photolabile analyte through photoreversion in fluorescence detector

Masaki Nishijima, Takehiko Wada, Koushi Nagamori, Yoshihisa Inoue

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Ultrahigh sensitivity chiral HPLC analysis of nonfluorescent cyclodimers of 2-anthracenecarboxylic acid (AC) was achieved with much improved accuracy and reproducibility through a nonconventional on-detector photoreversion/re- excitation/detection mechanism. Excitation of cyclodimers at 254 nm in the detector cuvette caused photoreversion to AC, in situ excitation of which led to strong fluorescence at longer λ. The sensitivity was enhanced by a factor of ca. 2000 compared to usual UV detection.

本文言語English
ページ(範囲)726-727
ページ数2
ジャーナルChemistry Letters
38
7
DOI
出版ステータスPublished - 2009 7 20

ASJC Scopus subject areas

  • 化学 (全般)

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