High-resolution pH imaging sensor for microscopic observation of microorganisms

Motoi Nakao, Shinji Inoue, Tatsuo Yoshinobu, Hiroshi Iwasaki

研究成果: Article査読

84 被引用数 (Scopus)

抄録

We have constructed a high-resolution pH imaging sensor, which enables one to observe the metabolic activity of microorganisms microscopically. A spatial resolution better than 10 μm was achieved by thinning the Si substrate to 20 μm, and by employing an infrared laser as an illumination source. Using a 100 kHz digital sampling technique for signal detection, a pH image of 128 x 128 pixels was measured within 15 min. The pH imaging sensor was integrated into a system for microscopic observation of incubated biological specimens such as microorganisms. Using the pH imaging sensor, Escherichia coli (E. coli) colonies incubated on agar medium for 8 h (visual size ∼0.2 mm diameter, number of cells ∼800) were detected. The observed pH distribution was in agreement with the result of simulation based on the production and diffusion of acidic ions.

本文言語English
ページ(範囲)234-239
ページ数6
ジャーナルSensors and Actuators, B: Chemical
34
1-3
DOI
出版ステータスPublished - 1996 8
外部発表はい

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

フィンガープリント 「High-resolution pH imaging sensor for microscopic observation of microorganisms」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル