High prevalence of extended-spectrum β-lactamases and qnr determinants in citrobacter species from japan: Dissemination of CTX-M-2

Hajime Kanamori, Hisakazu Yano, Yoichi Hirakata, Shiro Endo, Kazuaki Arai, Miho Ogawa, Masahiro Shimojima, Tetsuji Aoyagi, Masumitsu Hatta, Mitsuhiro Yamada, Katsushi Nishimaki, Miho Kitagawa, Hiroyuki Kunishima, Mitsuo Kaku

研究成果: Article査読

30 被引用数 (Scopus)

抄録

Objectives: Extended-spectrum β-lactamases (ESBLs) have become a problem among AmpC-producing Enterobacteriaceae and the emergence of concomitant quinolone resistance in β-lactamase-producing isolates poses a global threat. In this study we investigated the prevalence and regional variation of ESBLs in Japanese clinical isolates of Citrobacter spp. and analysed plasmid-mediated quinolone resistance (PMQR) determinants in ESBL-producing Citrobacter spp. Methods: A total of 348 clinical isolates of Citrobacter spp. collected throughout Japan were studied. Screening and the boronic acid disc test were performed to detect ESBLs in Citrobacter spp. with chromosomal AmpC β-lactamases. PCR and sequencing were done to identify ESBL and PMQR genes. For ESBL-producing Citrobacter spp., PFGE was performed using the SfiI restriction enzyme. Results: The number of ESBL-producing isolates confirmed phenotypically was 67 (19.3%). The prevalence of ESBL-producing Citrobacter koseri was significantly higher (32.1%) than that of ESBL-producing Citrobacter freundii (4.6%) (P < 0.01). Moreover, the prevalence of ESBLs was notably higher among C. koseri from southern Japan (60.0%). CTX-M-2 was predominant in C. koseri. Of the ESBL-producing C. koseri analysed, 23.2% possessed PMQR determinants, and there was a significant association between qnrB4 and bla SHV-12. The 57 ESBL-producing Citrobacter spp. possessing bla CTX-M, bla SHV or bla TEM were divided into 18 unique PFGE types. Conclusions: This is the first report about the prevalence of PMQR determinants among ESBL-producing Citrobacter spp. from Japan. Our data suggest that ESBLs and PMQR determinants are spreading among C. koseri in Japan.

本文言語English
論文番号dkr283
ページ(範囲)2255-2262
ページ数8
ジャーナルJournal of Antimicrobial Chemotherapy
66
10
DOI
出版ステータスPublished - 2011 10

ASJC Scopus subject areas

  • 薬理学
  • 微生物学(医療)
  • 感染症
  • 薬理学(医学)

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