High-performance liquid chromatography with photodiode array detection for determination of nobiletin content in the brain and serum of mice administrated the natural compound

Daisuke Saigusa, Masatoshi Shibuya, Daisuke Jinno, Hiroyuki Yamakoshi, Yoshiharu Iwabuchi, Akihito Yokosuka, Yoshihiro Mimaki, Akira Naganuma, Yasushi Ohizumi, Yoshihisa Tomioka, Tohru Yamakuni

研究成果: Article査読

38 被引用数 (Scopus)

抄録

We recently demonstrated that nobiletin, a citrus flavonoid, exhibits anti-dementia action in animals. However, no determination methods for the content of nobiletin with beneficial action in the brain of nobiletin- administered animals have been developed, nor has its pharmacokinetics been revealed completely. Here, we established the high-performance liquid chromatography/photodiode array detection method for nobiletin determination using Bond Elut C18 SPE cartridges for extraction, where the calibration curve was linear over 0.025-10 ng, with coefficient of variation of less than 6.76%. This method enabled us to determine pharmacokinetic parameters of nobiletin given intraperitoneally or per os in the brain of mice.

本文言語English
ページ(範囲)3635-3641
ページ数7
ジャーナルAnalytical and Bioanalytical Chemistry
400
10
DOI
出版ステータスPublished - 2011 7

ASJC Scopus subject areas

  • 分析化学
  • 生化学

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