High Performance Liquid Chromatography of Fluorescein Isothiocyanate Isomers and Intermediate Products

Koji Muramoto, Hisao Kamiya, Hiroshi Kawauchi, Hiroshi Meguro

    研究成果: Article査読

    3 被引用数 (Scopus)

    抄録

    Fluorescein isothiocyanate (FITC) isomers and the intermediate products have been separated by reversed phase high performance liquid chromatography (HPLC), using a linear gradient formed from acetone and 0,01 M sodium phosphate buffer (pH 7.0) or an isocratic elution of 40% acetone in 0.1 M pyridine-acetic acid (pH 7,0). This HPLC system was used to evaluate commercial FITC products. A method for the purification of the FITC product by HPLC was also described.

    本文言語English
    ページ(範囲)447-450
    ページ数4
    ジャーナルanalytical sciences
    1
    5
    DOI
    出版ステータスPublished - 1985

    ASJC Scopus subject areas

    • 分析化学

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