High-Performance Liquid Chromatography of Estrogen 3-Sulfates with Electrochemical Detection Using an Immobilized Enzyme Reactor

Toshifumi Niwa, Tomoyoshi Koshiyama, Shunji Ishiwata, Junichi Goto, Toshio Nambara

研究成果: Article査読

1 被引用数 (Scopus)

抄録

A simple and sensitive method for the determination of estrogen 3-sulfates by high-performance liquid chromatography with electrochemical detection is described. The sulfates were separated on a reversed-phase column and subsequently hydrolyzed with an immobilized sulfatase reactor in a continuous flow mode. Estrogens liberated were then detected with a coulometric detector. A linear response was observed for four sulfates, estrone sulfate, 16 α - hydroxyestrone 3-sulfate, estradiol 3-sulfate and estriol 3-sulfate in the range 25-1000 ng as an injected amount. The detection limits (S/N = 10) were 20 ng for estriol 3-sulfate and 16 α -hydroxyestrone 3-sulfate, and 50 ng for estrone sulfate and estradiol 3-sulfate, respectively.

本文言語English
ページ(範囲)242-246
ページ数5
ジャーナルJapanese Journal of Clinical Chemistry
20
4
DOI
出版ステータスPublished - 1991 1 1

ASJC Scopus subject areas

  • Clinical Biochemistry

フィンガープリント 「High-Performance Liquid Chromatography of Estrogen 3-Sulfates with Electrochemical Detection Using an Immobilized Enzyme Reactor」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル