High-Performance Liquid Chromatographic Method for the Analysis of Nτ-Methylhistidine in Food, Chicken Excreta, and Rat Urine

Kunioki Hayashi, Yoshizane Maeda, Masaaki Toyomizu, Yuichiro Tomita

研究成果: Article査読

35 被引用数 (Scopus)

抄録

A reliable high-performance liquid chromatographic method that utilizes a reversed-phase separation with ion-pairing and post-column fluorescence derivatization for the analysis of Nτ-methylhistidine in food, chicken excreta, and rat urine is described. Nτ-Methylhistidine in the hydrolyzed sample is first roughly separated from acid and neutral amino acids by an ion-exchange column. The Nτ-methylhistidine fraction is then evaporated and the residue is dissolved in the mobile phase (15 mM sodium octane sulfonate in 20 mM potassium phosphate), and subjected to high-performance liquid chromatography.

本文言語English
ページ(範囲)151-156
ページ数6
ジャーナルJournal of nutritional science and vitaminology
33
2
DOI
出版ステータスPublished - 1987

ASJC Scopus subject areas

  • 医学(その他)
  • 栄養および糖尿病

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