High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 are significant target antigens of perinuclear anti-neutrophil cytoplasmic antibodies in autoimmune hepatitis

J. Sobajima, S. Ozaki, H. Uesugi, F. Osakada, M. Inoue, Y. Fukuda, H. Shirakawa, M. Yoshida, A. Rokuhara, H. Imai, K. Kiyosawa, K. Nakao

研究成果: Article査読

63 被引用数 (Scopus)

抄録

Background - High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 have been identified as novel antigens of perinuclear anti- neutrophil cytoplasmic antibodies (pANCAs), and the existence of anti-HMG1 and anti-HMG2 antibodies in a population of patients with ulcerative colitis has been reported. Aims - To investigate whether HMG1 and HMG2 are target antigens for p-ANCAs in autoimmune hepatitis (AIH). Patients - Serum samples from 28 patients with AIH, 44 patients with primary biliary cirrhosis (PBC), 27 patients with chronic hepatitis C, and 23 patients with chronic hepatitis B were tested. Methods - ANCAs were detected by routine indirect immunofluorescence (IIF). Anti-HMG1 and anti-HMG2 antibodies were assayed by enzyme linked immunosorbent assay. Results - p-ANCAs were detected in 89% (25/28) of patients with AIH, 36% (16/44) of patients with PBC, 11% (3/27) of patients with chronic hepatitis C, and 13% (3/23) of patients with chronic hepatitis B. Anti-HMG1 and/or anti-HMG2 antibodies were detected in 89% (25/28) of patients with AIH, 70% (31/44) with PBC, 26% (7/27) with chronic hepatitis C, and 9% (2/23) with chronic hepatitis B. In AIH, anti-HMG1 and/or anti-HMG2 antibodies were detected in 96% (24/25) of p-ANCA positive patients. The p-ANCA staining pattern detected by IIF using sera from patients with AIH disappeared or decreased in titre after preincubation with a mixture of HMG1/HMG2. The presence and titres of those antibodies in AIH correlated significantly with those of p-ANCA, but not with those of antinuclear antibody or anti-smooth muscle antibody. Conclusions - HMG1 and HMG2 are significant target antigens of p-ANCA in AIH.

本文言語English
ページ(範囲)867-873
ページ数7
ジャーナルGut
44
6
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 消化器病学

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