High-level expression and characterization of fully active recombinant congereel galectins in eschericia coli

Tomohisa Ogawa, Chihiro Ishii, Yuji Suda, Hisao Kamiya, Koji Muramoto

研究成果: Article

11 引用 (Scopus)

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An expression system for recombinant conger eel galectins, congerins I and II, were constructed using the pTV 118N plasmid vector and Escherichia coli.Recombinant congerins I and II could be obtained in the soluble active form with high quantitative yield. Mutation of codons for Val andLeu located in the N-terminal region of Con I increased the expression efficiency. Purification of recombinant proteins were done by only two chromatographical steps from E. coli extract. The purified recombinant congerins were found to be almost the same as the native ones except for the acetyl group at the N-terminus; that is, they showed the same structures and carbohydrate binding activities, suggesting that N-terminal acetyl groups of congerins were not significant for activity.

元の言語English
ページ(範囲)476-480
ページ数5
ジャーナルBioscience, Biotechnology and Biochemistry
66
発行部数2
DOI
出版物ステータスPublished - 2002

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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