Heterologous expression of aspergillus oryzae xylose reductase and xylitol dehydrogenase genes facilitated xylose utilization in the yeast saccharomyces cerevisiae

Jitsuro Kaneda, Kenji Sasaki, Katsuya Gomi, Takahiro Shintani

研究成果: Article査読

2 被引用数 (Scopus)

抄録

abs The cDNA encoding a putative xylose reductase (xyrA) from Aspergillus oryzae was cloned and coexpressed in the yeast Saccharomyces cerevisiae with A. oryzae xylitol dehydrogenase cDNA (xdhA). XyrA exhibited NADPH-dependent xylose reductase activity. The S. cerevisiae strain, overexpressing the xyrA, xdhA, endogenous XKS1, and TAL1 genes, grew on xylose as sole carbon source, and produced ethanol.

本文言語English
ページ(範囲)168-170
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
75
1
DOI
出版ステータスPublished - 2011 1 28

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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