TY - JOUR
T1 - Hepatocyte growth factor induces angiogenesis in injured lungs through mobilizing endothelial progenitor cells
AU - Ishizawa, Kota
AU - Kubo, Hiroshi
AU - Yamada, Mitsuhiro
AU - Kobayashi, Seiichi
AU - Suzuki, Takashi
AU - Mizuno, Shinya
AU - Nakamura, Toshikazu
AU - Sasaki, Hidetada
N1 - Funding Information:
This work was supported by a grant from the Japan Society for the Promotion of Science 15590792 to H.K. We thank Prof. Masaru Okabe (Genome Information Research Centre, Osaka University, Japan) for providing GFP transgenic mice (C57BL/6 TgN(act-GFP)OsbC14-Y01-FM131).
PY - 2004/11/5
Y1 - 2004/11/5
N2 - Circulating endothelial progenitor cells (EPCs) play a pivotal role in angiogenesis. Hepatocyte growth factor (HGF) is known to induce proliferation and motility in endothelial cells, and to play a role in mitogenic and morphogenic actions. However, the role of HGF in EPC mobilization has not been clearly described yet. We investigated the effect of HGF on mobilizing EPCs and on angiogenesis in elastase-induced lung injury. HGF significantly increased the triple-positive (Sca-1 +, Flk-1 +, and c-kit +) fraction in peripheral mononuclear cells in mice. The bone marrow-derived cells were recruited into the injured lungs, where they differentiated to capillary endothelial cells. HGF induced proliferation of both bone marrow-derived and resident endothelial cells in the alveolar wall. In conclusion, the present study suggests that HGF induces EPC mobilization from the bone marrow and enhances the proliferation of endothelial cells in vivo. These complex effects induced by HGF orchestrate pulmonary regeneration in emphysematous lung parenchyma.
AB - Circulating endothelial progenitor cells (EPCs) play a pivotal role in angiogenesis. Hepatocyte growth factor (HGF) is known to induce proliferation and motility in endothelial cells, and to play a role in mitogenic and morphogenic actions. However, the role of HGF in EPC mobilization has not been clearly described yet. We investigated the effect of HGF on mobilizing EPCs and on angiogenesis in elastase-induced lung injury. HGF significantly increased the triple-positive (Sca-1 +, Flk-1 +, and c-kit +) fraction in peripheral mononuclear cells in mice. The bone marrow-derived cells were recruited into the injured lungs, where they differentiated to capillary endothelial cells. HGF induced proliferation of both bone marrow-derived and resident endothelial cells in the alveolar wall. In conclusion, the present study suggests that HGF induces EPC mobilization from the bone marrow and enhances the proliferation of endothelial cells in vivo. These complex effects induced by HGF orchestrate pulmonary regeneration in emphysematous lung parenchyma.
KW - Angiogenesis
KW - Bone marrow
KW - Endothelial progenitor cell
KW - Hepatocyte growth factor
KW - Pulmonary emphysema
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U2 - 10.1016/j.bbrc.2004.09.049
DO - 10.1016/j.bbrc.2004.09.049
M3 - Article
C2 - 15465014
AN - SCOPUS:4744364051
VL - 324
SP - 276
EP - 280
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -