TY - JOUR
T1 - Hemocytes of Ciona intestinalis express multiple genes involved in innate immune host defense
AU - Shida, Kazuhito
AU - Terajima, Daichi
AU - Uchino, Ryuji
AU - Ikawa, Shuntaro
AU - Ikeda, Makoto
AU - Asano, Katsutoshi
AU - Watanabe, Toshio
AU - Azumi, Kaoru
AU - Nonaka, Masaru
AU - Satou, Yutaka
AU - Satoh, Nori
AU - Satake, Masanobu
AU - Kawazoe, Yoshiyuki
AU - Kasuya, Atsuo
PY - 2003/1/10
Y1 - 2003/1/10
N2 - Ascidians, which are classified as urochordata, appear to employ a primitive system of host defense that is considered to be a prototype of vertebrate innate immunity. We performed a cDNA/EST study to identify the genes expressed in the hemocytes of Ciona intestinalis. We obtained 3357 one-path reads that were then grouped into 1889 independent clusters. Although two thirds of the clusters could not be assigned to any particular gene, the remaining 530 clusters had significant homology to genes with known function. Of these, 62 clusters appeared to be related to host defense mechanisms. These include transcripts whose products are probably involved in cytotoxicity, detoxification, inflammation, and apoptosis. As expected, elements of acquired immunity were not detected. Thus, Ciona hemocytes appear to express a number of host defense-related genes involved in innate immune mechanisms.
AB - Ascidians, which are classified as urochordata, appear to employ a primitive system of host defense that is considered to be a prototype of vertebrate innate immunity. We performed a cDNA/EST study to identify the genes expressed in the hemocytes of Ciona intestinalis. We obtained 3357 one-path reads that were then grouped into 1889 independent clusters. Although two thirds of the clusters could not be assigned to any particular gene, the remaining 530 clusters had significant homology to genes with known function. Of these, 62 clusters appeared to be related to host defense mechanisms. These include transcripts whose products are probably involved in cytotoxicity, detoxification, inflammation, and apoptosis. As expected, elements of acquired immunity were not detected. Thus, Ciona hemocytes appear to express a number of host defense-related genes involved in innate immune mechanisms.
KW - Ascidian
KW - EST
KW - Hemocytes
KW - Host defense
KW - Innate immunity
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U2 - 10.1016/S0006-291X(03)00113-X
DO - 10.1016/S0006-291X(03)00113-X
M3 - Article
C2 - 12604333
AN - SCOPUS:0037428258
VL - 302
SP - 207
EP - 218
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -