Hedgehog-Gli activators direct osteo-chondrogenic function of bone morphogenetic protein toward osteogenesis in the perichondrium

Hironori Hojo, Shinsuke Ohba, Kiyomi Taniguchi, Masataka Shirai, Fumiko Yano, Taku Saito, Toshiyuki Ikeda, Keiji Nakajima, Yuske Komiyama, Naomi Nakagata, Kentaro Suzuki, Yuji Mishina, Masahisa Yamada, Tomohiro Konno, Tsuyoshi Takato, Hiroshi Kawaguchi, Hideki Kambara, Ung Il Chung

研究成果: Article査読

45 被引用数 (Scopus)

抄録

Specification of progenitors into the osteoblast lineage is an essential event for skeletogenesis. During endochondral ossification, cells in the perichondrium give rise to osteoblast precursors. Hedgehog (Hh) and bone morphogenetic protein (BMP) are suggested to regulate the commitment of these cells. However, properties of perichondrial cells and regulatory mechanisms of the specification process are still poorly understood. Here, we investigated the machineries by combining a novel organ culture system and single-cell expression analysis with mouse genetics and biochemical analyses. In a metatarsal organ culture reproducing bone collar formation, activation of BMP signaling enhanced the bone collar formation cooperatively with Hh input, whereas the signaling induced ectopic chondrocyte formation in the perichondrium without Hh input. Similar phenotypes were also observed in compound mutant mice, where signaling activities of Hh and BMP were genetically manipulated. Single-cell quantitative RT-PCR analyses showed heterogeneity of perichondrial cells in terms of natural characteristics and responsiveness to Hh input. In vitro analyses revealed that Hh signaling suppressed BMP-induced chondrogenic differentiation; Gli1 inhibited the expression of Sox5, Sox6, and Sox9 (SRY box-containing gene 9) as well as transactivation by Sox9. Indeed, ectopic expression of chondrocyte maker genes were observed in the perichondrium of metatarsals in Gli1-/- fetuses, and the phenotype was more severe in Gli1-/-;Gli2-/- newborns. These data suggest that Hh-Gli activators alter the function of BMP to specify perichondrial cells into osteoblasts; the timing of Hh input and its target populations are critical for BMP function.

本文言語English
ページ(範囲)9924-9932
ページ数9
ジャーナルJournal of Biological Chemistry
288
14
DOI
出版ステータスPublished - 2013 4 5
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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