Hadp1, a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish

Joshua D. Wythe, Michael J. Jurynec, Lisa D. Urness, Christopher A. Jones, M. Khaled Sabeh, Andreas A. Werdich, Mariko Sato, H. Joseph Yost, David J. Grunwald, Calum A. MacRae, Dean Y. Li

研究成果: Article査読

15 被引用数 (Scopus)

抄録

The vertebrate heart is one of the first organs to form, and its early function and morphogenesis are crucial for continued embryonic development. Here we analyze the effects of loss of Heart adaptor protein 1 (Hadp1), which we show is required for normal function and morphogenesis of the embryonic zebrafish heart. Hadp1 is a pleckstrin homology (PH)-domain-containing protein whose expression is enriched in embryonic cardiomyocytes. Knockdown of hadp1 in zebrafish embryos reduced cardiac contractility and altered late myocyte differentiation. By using optical mapping and submaximal levels of hadp1 knockdown, we observed profound effects on Ca 2+ handling and on action potential duration in the absence of morphological defects, suggesting that Hadp1 plays a major role in the regulation of intracellular Ca 2+ handling in the heart. Hadp1 interacts with phosphatidylinositol 4-phosphate [PI4P; also known as PtdIns(4)P] derivatives via its PH domain, and its subcellular localization is dependent upon this motif. Pharmacological blockade of the synthesis of PI4P derivatives in vivo phenocopied the loss of hadp1 in zebrafish. Collectively, these results demonstrate that hadp1 is required for normal cardiac function and morphogenesis during embryogenesis, and suggest that hadp1 modulates Ca 2+ handling in the heart through its interaction with phosphatidylinositols.

本文言語English
ページ(範囲)607-621
ページ数15
ジャーナルDMM Disease Models and Mechanisms
4
5
DOI
出版ステータスPublished - 2011 9

ASJC Scopus subject areas

  • 神経科学(その他)
  • 医学(その他)
  • 免疫学および微生物学(その他)
  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「Hadp1, a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル