Grease matrix as a versatile carrier of proteins for serial crystallography

Michihiro Sugahara; Eiichi Mizohata; Eriko Nango; Mamoru Suzuki; Tomoyuki Tanaka; Tetsuya Masuda; Rie Tanaka; Tatsuro Shimamura; Yoshiki Tanaka; Chiyo Suno; Kentaro Ihara; Dongqing Pan; Keisuke Kakinouchi; Shigeru Sugiyama; Michio Murata; Tsuyoshi Inoue; Kensuke Tono; Changyong Song; Jaehyun Park; Takashi Kameshima; Takaki Hatsui; Yasumasa Joti; Makina Yabashi; So Iwata

doi:10.1038/nmeth.3172

Grease matrix as a versatile carrier of proteins for serial crystallography

Michihiro Sugahara, Eiichi Mizohata, Eriko Nango, Mamoru Suzuki, Tomoyuki Tanaka, Tetsuya Masuda, Rie Tanaka, Tatsuro Shimamura, Yoshiki Tanaka, Chiyo Suno, Kentaro Ihara, Dongqing Pan, Keisuke Kakinouchi, Shigeru Sugiyama, Michio Murata, Tsuyoshi Inoue, Kensuke Tono, Changyong Song, Jaehyun Park, Takashi KameshimaTakaki Hatsui, Yasumasa Joti, Makina Yabashi, So Iwata

研究成果: Article › 査読

158 被引用数 (Scopus)

抄録

Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

本文言語	English
ページ（範囲）	61-63
ページ数	3
ジャーナル	Nature Methods
巻	12
号	1
DOI	https://doi.org/10.1038/nmeth.3172
出版ステータス	Published - 2014 1月 1
外部発表	はい

ASJC Scopus subject areas

バイオテクノロジー
生化学
分子生物学
細胞生物学

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10.1038/nmeth.3172

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Sugahara, M., Mizohata, E., Nango, E., Suzuki, M., Tanaka, T., Masuda, T., Tanaka, R., Shimamura, T., Tanaka, Y., Suno, C., Ihara, K., Pan, D., Kakinouchi, K., Sugiyama, S., Murata, M., Inoue, T., Tono, K., Song, C., Park, J., ... Iwata, S. (2014). Grease matrix as a versatile carrier of proteins for serial crystallography. Nature Methods, 12(1), 61-63. https://doi.org/10.1038/nmeth.3172

Sugahara, M, Mizohata, E, Nango, E, Suzuki, M, Tanaka, T, Masuda, T, Tanaka, R, Shimamura, T, Tanaka, Y, Suno, C, Ihara, K, Pan, D, Kakinouchi, K, Sugiyama, S, Murata, M, Inoue, T, Tono, K, Song, C, Park, J, Kameshima, T, Hatsui, T, Joti, Y, Yabashi, M & Iwata, S 2014, 'Grease matrix as a versatile carrier of proteins for serial crystallography', Nature Methods, vol. 12, no. 1, pp. 61-63. https://doi.org/10.1038/nmeth.3172

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abstract = "Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 {\AA}.",

author = "Michihiro Sugahara and Eiichi Mizohata and Eriko Nango and Mamoru Suzuki and Tomoyuki Tanaka and Tetsuya Masuda and Rie Tanaka and Tatsuro Shimamura and Yoshiki Tanaka and Chiyo Suno and Kentaro Ihara and Dongqing Pan and Keisuke Kakinouchi and Shigeru Sugiyama and Michio Murata and Tsuyoshi Inoue and Kensuke Tono and Changyong Song and Jaehyun Park and Takashi Kameshima and Takaki Hatsui and Yasumasa Joti and Makina Yabashi and So Iwata",

note = "Funding Information: The XFEL experiments were carried out at the BL3 of SACLA with the approval of the Japan Synchrotron Radiation Research Institute (JASRI) (proposal nos. 2012B8036, 2013A8039, 2013A8040, 2013B8044, 2013B8045 and 2014A8032). This work was supported by RIKEN, by the X-ray Free-Electron Laser Priority Strategy Program (MEXT) and partly by Research Acceleration Program of Japan Science and Technology Agency. The sample preparation of FABP3 was supported by the JST-ERATO Murata Lipid Active Structure Project. The authors thank the SACLA beamline staff for technical assistance, K. Diederichs for help with data analysis and A. Nisbet for careful reading of the manuscript.",

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AU - Mizohata, Eiichi

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AU - Masuda, Tetsuya

AU - Tanaka, Rie

AU - Shimamura, Tatsuro

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AU - Suno, Chiyo

AU - Ihara, Kentaro

AU - Pan, Dongqing

AU - Kakinouchi, Keisuke

AU - Sugiyama, Shigeru

AU - Murata, Michio

AU - Inoue, Tsuyoshi

AU - Tono, Kensuke

AU - Song, Changyong

AU - Park, Jaehyun

AU - Kameshima, Takashi

AU - Hatsui, Takaki

AU - Joti, Yasumasa

AU - Yabashi, Makina

AU - Iwata, So

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PY - 2014/1/1

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Grease matrix as a versatile carrier of proteins for serial crystallography

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