Grease matrix as a versatile carrier of proteins for serial crystallography

Michihiro Sugahara, Eiichi Mizohata, Eriko Nango, Mamoru Suzuki, Tomoyuki Tanaka, Tetsuya Masuda, Rie Tanaka, Tatsuro Shimamura, Yoshiki Tanaka, Chiyo Suno, Kentaro Ihara, Dongqing Pan, Keisuke Kakinouchi, Shigeru Sugiyama, Michio Murata, Tsuyoshi Inoue, Kensuke Tono, Changyong Song, Jaehyun Park, Takashi KameshimaTakaki Hatsui, Yasumasa Joti, Makina Yabashi, So Iwata

研究成果: Article査読

158 被引用数 (Scopus)

抄録

Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

本文言語English
ページ(範囲)61-63
ページ数3
ジャーナルNature Methods
12
1
DOI
出版ステータスPublished - 2014 1月 1
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生化学
  • 分子生物学
  • 細胞生物学

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