TY - JOUR
T1 - Gonadal estrogen profile and immunohistochemical localization of steroidogenic enzymes in the oyster and scallop during sexual maturation
AU - Matsumoto, Toshie
AU - Osada, Makoto
AU - Osawa, Yoshio
AU - Mori, Katsuyoshi
N1 - Funding Information:
The authors are grateful to the staff of the Education and Research Center of Marine Bio-Sciences (ERCMB) of Tohoku University, for their help in our experiment. We also thank Carol Yarborough and Margaret Cegielski of Hauptman-Woodward Medical Research Institute for reviewing the manuscript, and Miss. Mii Tachibana of Tohoku College of Pharmacy, Sendai, for her excellent technical assistance in the aromatase activity assay. This work was supported by Grant-in-Aid for Scientific Research (C) (No. 07660231) from the Japanese Ministry of Education, Science and Culture (M. Osada) and in part by USPHS Research Grant HD04945 (Y. Osawa).
PY - 1997
Y1 - 1997
N2 - Estrogen levels in the gonads of marine bivalves, the Pacific oyster Crassostrea gigas and scallop Patinopecten yessoensis were determined by high performance liquid chromatography (HPLC) using an electrochemical detector. Estrone (E1), estradiol-17β (E2), and a small amount of estriol (E3) were identified in the ovary, while only E2 was found in the testis. The level of E2 in the ovary was consistently higher than E1 and it increased with sexual maturation. These results indicate that E2 may play a role in the reproductive events of the oyster and scallop. In vitro experiments demonstrated the presence of 17β-hydroxysteroid dehydrogenase (17β-HSD) in the ovaries of both bivalves. The activity of 17β-HSD in the ovary was lower in the postspawning stage than in the early differentiating stage. The evidence for the presence of aromatase activity in the scallop ovary was obtained by 3H-water assay. The immunoreactivity against 3β-hydroxysteroid dehydrogenase (3βHSD), P450 aromatase and E2 was detected in the cells along the outside of germinal acini of the scallop ovary. It is concluded that estrogens can be synthesized in the gonad, that their levels vary with the reproductive cycle, and that they have a role in the development of gametes.
AB - Estrogen levels in the gonads of marine bivalves, the Pacific oyster Crassostrea gigas and scallop Patinopecten yessoensis were determined by high performance liquid chromatography (HPLC) using an electrochemical detector. Estrone (E1), estradiol-17β (E2), and a small amount of estriol (E3) were identified in the ovary, while only E2 was found in the testis. The level of E2 in the ovary was consistently higher than E1 and it increased with sexual maturation. These results indicate that E2 may play a role in the reproductive events of the oyster and scallop. In vitro experiments demonstrated the presence of 17β-hydroxysteroid dehydrogenase (17β-HSD) in the ovaries of both bivalves. The activity of 17β-HSD in the ovary was lower in the postspawning stage than in the early differentiating stage. The evidence for the presence of aromatase activity in the scallop ovary was obtained by 3H-water assay. The immunoreactivity against 3β-hydroxysteroid dehydrogenase (3βHSD), P450 aromatase and E2 was detected in the cells along the outside of germinal acini of the scallop ovary. It is concluded that estrogens can be synthesized in the gonad, that their levels vary with the reproductive cycle, and that they have a role in the development of gametes.
KW - 17β-hydroxysteroid dehydrogenase
KW - 3β-hydroxysteroid dehydrogenase
KW - Estrogen
KW - Immunohistochemistry
KW - Oyster
KW - P450 aromatase
KW - Scallop
KW - Sexual maturation
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U2 - 10.1016/S0305-0491(97)00233-2
DO - 10.1016/S0305-0491(97)00233-2
M3 - Article
AN - SCOPUS:0031406314
VL - 118
SP - 811
EP - 817
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 4
ER -