Gold nanoparticles used as a carrier enhance production of anti-hapten IgG in rabbit: A study with azobenzene-dye as a hapten presented on the entire surface of gold nanoparticles

Noriyuki Ishii, Fitri Fitrilawati, Abhijit Manna, Haruhisa Akiyama, Yasushi Tamada, Kaoru Tamada

研究成果: Article査読

18 被引用数 (Scopus)

抄録

The azobenzene moiety, well-known not only for its reversible cis-to-trans photoisomerization but also as a hapten, does not induce antibodies on its own, but it reacts with antibodies raised against conjugates with protein carriers. Hence we selected azobenzene dye as an indicator to assess the possibility of having gold nano-particles act as an immunological carrier instead of protein carriers. In rabbits, we confirmed an in vivo response against azobenzene dye presented on the entire surface of gold nanoparticles (azo-nanoparticles), where the gold nanoparticles appeared to play a role as a carrier for the hapten. A high yield of immunoglobulin G (IgG) against the azobenzene derivative took place in rabbits injected with azo-nanoparticles, whereas no increase in IgG was recognized in other rabbits treated solely with chemically equivalent azobenzene dye instead of azo-nanoparticles. Electron microscopy and surface plasmon resonance spectroscopy indicated that the IgG obtained specifically recognized the difference between the isomer conformations of the azobenzene moiety.

本文言語English
ページ(範囲)124-131
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
72
1
DOI
出版ステータスPublished - 2008
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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