It is well known that protected acyclic sugar carbonates are useful glycosyl donors for chemical glycosylation reaction. Cyclic sugar derivatives, 1,2-(9-carbonylglycopyranoses(glucose carbonate 1), are potential glycosyl donors for glycosidase-catalyzed glycosylation reaction. Here we report the synthesis of glucose carbonate and the screening of hydrolases that recognize this compound. We synthesized the glucose carbonate from D-glucose via three steps. The enzymatic activity was found in some enzyme preparations, such as Sumizyme MC, from Rhizopus sp. in basic media, and in some α-glucosidase preparations from Rhizopus sp. and Bacillus sp. in acidic media.
|出版ステータス||Published - 2005 12月 1|
|イベント||54th SPSJ Symposium on Macromolecules - Yamagata, Japan|
継続期間: 2005 9月 20 → 2005 9月 22
|Other||54th SPSJ Symposium on Macromolecules|
|Period||05/9/20 → 05/9/22|
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