TY - JOUR
T1 - Glucose and glutamine metabolism in oral squamous cell carcinoma
T2 - Insight from a quantitative metabolomic approach
AU - Ogawa, Tamaki
AU - Washio, Jumpei
AU - Takahashi, Tetsu
AU - Echigo, Seishi
AU - Takahashi, Nobuhiro
N1 - Funding Information:
This study was supported by Grants-in-Aid for Scientific Research (Nos. 22390399 , 23659866 , and 25670786 ) and for young Scientists (B) (No. 23792498 ) from the Japan Society for the Promotion of Science , and by Research and Education Funding for the Inter-University Research Project (2007-2011), Ministry of Education, Culture, Sports, Science, and Technology, Japan .
PY - 2014/8
Y1 - 2014/8
N2 - Objective To characterize the metabolic system of oral squamous cell carcinoma (OSCC) by metabolome analysis. Study Design The metabolome profiles, including the Embden-Meyerhof-Parnas pathway (EMPP), the pentose phosphate pathway, the tricarboxylic acid cycle (TCAC), and amino acids, were obtained from OSCC and its surrounding normal tissues (32 patients) using capillary electrophoresis and a time-of-flight mass spectrometer. Results Enhancement of glucose consumption and lactate production (Warburg effect) was observed in OSCC tissues. The decrease of glucose along with the decrease of the downstream intermediates in the EMPP suggests that incorporated glucose is mainly consumed for biosynthesis. Glutamine consumption with the increase of the intermediates in the last half of the TCAC suggests the involvement of glutaminolysis, in which glutamine is converted to lactate via the last half of the TCAC. Conclusions It is suggested that OSCC tissues show the Warburg effect, which stems from the combined enhancement of glucose consumption and glutaminolysis.
AB - Objective To characterize the metabolic system of oral squamous cell carcinoma (OSCC) by metabolome analysis. Study Design The metabolome profiles, including the Embden-Meyerhof-Parnas pathway (EMPP), the pentose phosphate pathway, the tricarboxylic acid cycle (TCAC), and amino acids, were obtained from OSCC and its surrounding normal tissues (32 patients) using capillary electrophoresis and a time-of-flight mass spectrometer. Results Enhancement of glucose consumption and lactate production (Warburg effect) was observed in OSCC tissues. The decrease of glucose along with the decrease of the downstream intermediates in the EMPP suggests that incorporated glucose is mainly consumed for biosynthesis. Glutamine consumption with the increase of the intermediates in the last half of the TCAC suggests the involvement of glutaminolysis, in which glutamine is converted to lactate via the last half of the TCAC. Conclusions It is suggested that OSCC tissues show the Warburg effect, which stems from the combined enhancement of glucose consumption and glutaminolysis.
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U2 - 10.1016/j.oooo.2014.04.003
DO - 10.1016/j.oooo.2014.04.003
M3 - Article
C2 - 24927638
AN - SCOPUS:84904819009
SN - 2212-4403
VL - 118
SP - 218
EP - 225
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
IS - 2
ER -