Geranyl modification on the tryptophan residue of ComX RO-E-2 pheromone by a cell-free system

Fumitada Tsuji, Ayako Ishihara, Keiko Kurata, Aya Nakagawa, Masahiro Okada, Shigeyuki Kitamura, Kyoko Kanamaru, Yuichi Masuda, Kazuma Murakami, Kazuhiro Irie, Youji Sakagami

研究成果: Article査読

15 被引用数 (Scopus)

抄録

ComX pheromone is an isoprenoidal oligopeptide containing a modified tryptophan residue, which stimulates natural genetic competence in the gram-positive bacterium Bacillus. Since posttranslational prenylation on the tryptophan residue has not been reported except in ComX pheromone, the universality of this modification has not yet been elucidated. In this paper, we established a cell-free system, whereby the tryptophan residue in peptides is modified with a geranyl group by modifying enzyme ComQ. In addition, we investigated enzymatic reaction conditions using an in vitro enzyme reaction system. This is the first report of in vitro geranylation on the tryptophan residue. This system is potentially a useful tool for elucidating the universality of prenylation on the tryptophan residue.

本文言語English
ページ(範囲)174-179
ページ数6
ジャーナルFEBS Letters
586
2
DOI
出版ステータスPublished - 2012 1 20

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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