Genotyping of single nucleotide polymorphisms (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip.

Masahiro Hiratsuka, Aiko Ebisawa, Yoichi Matsubara, Shigeo Kure, Yumiko Konno, Takamitsu Sasaki, Michinao Mizugaki

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Using competitive allele-specific oligonucleotide hybridization with immunochromatographic strip (CASSOH), we have developed a simplified method for the detection of eight polymorphisms that are especially important in the identification of drug responders or non-responders and patients at increased risk of drug toxicity. The genotyping method is unambiguously determined by the presence or the absence of visible purple lines on the immunochromatographic strip, and results are obtained within 5 min after PCR. This method is rapid, highly sensitive, simplified, and should be suitable for point-of-care genotyping in clinical settings.

本文言語English
ページ(範囲)303-307
ページ数5
ジャーナルDrug metabolism and pharmacokinetics
19
4
DOI
出版ステータスPublished - 2004 8月
外部発表はい

ASJC Scopus subject areas

  • 薬理学
  • 薬科学
  • 薬理学(医学)

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