Genome-wide analyses of the structural gene families involved in the legume-specific 5-deoxyisoflavonoid biosynthesis of lotus japonicus

Norimoto Shimada, Shusei Sato, Tomoyoshi Akashi, Yasukazu Nakamura, Satoshi Tabata, Shin Ichi Ayabe, Toshio Aoki

研究成果: Article査読

33 被引用数 (Scopus)

抄録

A model legume Lotus japonicus (Regel) K. Larsen is one of the subjects of genome sequencing and functional genomics programs. In the course of targeted approaches to the legume genomics, we analyzed the genes encoding enzymes involved in the biosynthesis of the legume-specific 5-deoxyisoflavonoid of L. japonicus, which produces isoflavan phytoalexins on elicitor treatment. The paralogous biosynthetic genes were assigned as comprehensively as possible by biochemical experiments, similarity searches, comparison of the gene structures, and phylogenetic analyses. Among the 10 biosynthetic genes investigated, six comprise multigene families, and in many cases they form gene clusters in the chromosomes. Semi-quantitative reverse transcriptase-PCR analyses showed coordinate up-regulation of most of the genes during phytoalexin induction and complex accumulation patterns of the transcripts in different organs. Some paralogous genes exhibited similar expression specificities, suggesting their genetic redundancy. The molecular evolution of the biosynthetic genes is discussed. The results presented here provide reliable annotations of the genes and genetic markers for comparative and functional genomics of leguminous plants.

本文言語English
ページ(範囲)25-36
ページ数12
ジャーナルDNA Research
14
1
DOI
出版ステータスPublished - 2007 3月
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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