Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite Begomovirus

Tatsuya Kon, Lolita M. Dolores, Akiko Murayama, Narceo B. Bajet, Shu Hase, Hideki Takahashi, Masato Ikegami

    研究成果: Article査読

    22 被引用数 (Scopus)

    抄録

    Complete nucleotide sequence of infectious cloned DNA of Tomato leaf curl virus from Philippines (ToLCV-Ph) was determined. The single circular DNA molecule comprises 2755 nucleotides. ToLCV-Ph DNA contains six open reading frames (ORFs) each capable of encoding proteins with a molecular weight greater than 10 kDa. A partial dimeric ToLCV-Ph DNA clone was constructed in a binary vector and used to agroinoculate tomato plants (Lycopersicon esculentum Mill. cv. Zuikou 102). Typical leaf curl symptoms were observed, showing that the single DNA component is sufficient for infectivity. In total nucleotide sequence comparisons with other geminiviruses, ToLCV-Ph was most closely related to Ageratum yellow vein virus (AYVV) (79% identity), ToLCV-Laos (78%), Soyabean crinkle leaf virus-Thailand (78%) and ToLCV-Taiwan (77%). The significant but relatively low sequence identity in the genomic DNA between ToLCV-Ph and other geminiviruses suggests that it is a distinct geminivirus in the genus Begomovirus.

    本文言語English
    ページ(範囲)587-591
    ページ数5
    ジャーナルJournal of Phytopathology
    150
    11-12
    DOI
    出版ステータスPublished - 2002 12月 1

    ASJC Scopus subject areas

    • 生理学
    • 農業および作物学
    • 遺伝学
    • 植物科学

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