Genome mining approach for harnessing the cryptic gene cluster in Alternaria solani: Production of PKS-NRPS hybrid metabolite, didymellamide B

Takahiro Ugai, Atsushi Minami, Katsuya Gomi, Hideaki Oikawa

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Using a heterologous expression system in Aspergillus oryzae we characterized cryptic biosynthetic genes found in Alternaria solani. This system enabled the isolation of an antifungal metabolite, didymellamide B, which was previously not detected in the extract of the A. solani. The co-production and chemical transformation of didymellamide congeners enabled us to decipher the biosynthetic pathway of didymellamide B as follows: (1) formation of a linear polyketide chain with 3-acyltetramate, (2) oxidative ring expansion to yield a 2-pyridone skeleton, and (3) non-enzymatic endo-selective [4+2] cycloaddition to afford a trans-decalin. These results demonstrate the robustness and reliability of the A. oryzae expression system.

本文言語English
ページ(範囲)2793-2796
ページ数4
ジャーナルTetrahedron Letters
57
25
DOI
出版ステータスPublished - 2016 6 22

ASJC Scopus subject areas

  • 生化学
  • 創薬
  • 有機化学

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