Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect

Kanako Usuda, Yasuaki Wada, Yasuhiro Ishimaru, Takanori Kobayashi, Michiko Takahashi, Hiromi Nakanishi, Yasuo Nagato, Satoshi Mori, Naoko K. Nishizawa

研究成果: Article査読

35 被引用数 (Scopus)

抄録

Nicotianamine (NA), a metal chelator ubiquitous in higher plants, serves as an antihypertensive substance in humans. To engineer a novel antihypertensive rice that contains larger amounts of NA, the barley NA synthase gene, HvNAS1, was introduced into rice via Agrobacterium-mediated transformation. The introduced HvNAS1 was driven by pGluB-1, which induces strong gene expression in the endosperm of rice seeds. The NA content in transgenic rice seeds was up to fourfold greater than that in non-transgenic rice seeds. The Cre/loxP DNA excision (CLX) system was used to remove the selectable marker gene for antibiotic resistance. Furthermore, the transgenic rice was crossed with a cleistogamous mutant to prevent gene transfer via pollen dispersal. These two modifications may minimize public concern with regard to the use of this transgenic rice.

本文言語English
ページ(範囲)87-95
ページ数9
ジャーナルPlant Biotechnology Journal
7
1
DOI
出版ステータスPublished - 2009 1月
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 農業および作物学
  • 植物科学

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