Genetic Transformation in Helicobacter pylori

Masataka Tsuda, Mikio Karita, Teruo Nakazawa

研究成果: Article査読

42 被引用数 (Scopus)

抄録

Genetic transformation in Helicobacter pylori was investigated by using its chromosomal and plasmid DNAs. Six out of the eight strains exhibited the natural competence for incorporation of H. pylori chromosomal DNA, and all the strains incorporated the donor DNA efficiently by washing and concentrating the cells, with a glycerol solution. The much higher frequency of transformation was obtained in each strain by means of electroporation. Electroporation experiments were also conducted by use of the recombinant DNAs consisting of the H. pylori and Escherichia coli plasmids as the donors, and the occurrence of the homologous recombination was demonstrated between the incoming H. pylori plasmid‐derived region and the corresponding region of the originally residing plasmid in H. pylori.

本文言語English
ページ(範囲)85-89
ページ数5
ジャーナルMICROBIOLOGY and IMMUNOLOGY
37
1
DOI
出版ステータスPublished - 1993 1
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • ウイルス学

フィンガープリント

「Genetic Transformation in Helicobacter pylori」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル