Generation mechanism of radical species by tyrosine-tyrosinase reaction

Mika Tada, Masahiro Kohno, Shigenobu Kasai, Yoshimi Niwano

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Alleviated melanin formation in the skin through inhibition of tyrosine-tyrosinase reaction is one of the major targets of cosmetics for whitening ability. Since melanin has a pivotal role for photoprotection, there are pros and cons of inhibition of melanin formation. This study applying electron spin resonance (ESR)-spin trapping method revealed that .H and .OH are generated through tyrosine-tyrosinase reaction. When deuterium water was used instead of H2O, the signal of 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-H (a spin adduct of DMPO and .H) greatly decreased, whilst DMPO-OH (a spin adduct of DMPO and .OH) did not. Thus, it is suggested that .H was derived from H2O, and .OH through oxidative catalytic process of tyrosine to dopaquinone. Our study suggests that tyrosinase inhibitors might contribute to alleviate the oxidative damage of the skin by inhibiting .OH generation via the enzyme reaction.

本文言語English
ページ(範囲)162-166
ページ数5
ジャーナルJournal of Clinical Biochemistry and Nutrition
47
2
DOI
出版ステータスPublished - 2010 9

ASJC Scopus subject areas

  • 医学(その他)
  • 栄養および糖尿病
  • 臨床生化学

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