Generation mechanism of deferoxamine radical by tyrosine-tyrosinase reaction

Mika Tada, Yoshimi Niwano, Masahiro Kohno

研究成果: Article

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Nitroxide radical formations of deferoxamine mesylate (DFX) that is used clinically to treat iron-overload patients was examined by a tyrosine-tyrosinase reaction system as models of the H-atom transfer or proton-coupled electron transfer. When DFX was exposed to the tyrosine-tyrosinase reaction, nine-line ESR spectrum (g = 2.0063, hfcc; aN = 0.78 mT, aH(2) = 0.63 mT) was detected, indicating that the oxidation of DFX leads to a nitroxide radical. The signal intensity of the DFX radical increased dependently on the concentrations of tyrosine and tyrosinase. The amounts of DMPO-OH spin adducts via the tyrosine-tyrosinase reaction declined with DFX. Furthermore, mass spectra of an extra removed from the tyrosine-tyrosinase reaction mixture showed that the enzyme reactions might not be degradations of DFX. Therefore, there might be two types of DFX reaction passways, which could be through an internal electron transfer from tyrosine and hydrogen absorptions by .OH directly. 2015

元の言語English
ページ(範囲)911-916
ページ数6
ジャーナルanalytical sciences
31
発行部数9
DOI
出版物ステータスPublished - 2015 1 1

ASJC Scopus subject areas

  • Analytical Chemistry

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