Gene structure and expression of the targeting subunit, RGL, of the muscle-specific glycogen-associated type 1 protein phosphatase, PP1G

Carita Lannér, Yoichi Suzuki, Chen Bi Bi, Hong Zhang, Lori D. Cooper, Melissa M. Bowker-Kinley, A. A. DePaoli-Roach, Yoichi Suzuki

研究成果: Article査読

15 被引用数 (Scopus)

抄録

The type I phosphatase associated with glycogen, PP1G, plays an important role in glycogen metabolism. PP1G is targeted to glycogen by the RGL subunit, which regulates the function of the enzyme. We report the cloning and characterization of the gene as well as the pattern of expression of the RGL subunit from mouse. The gene covers more than 37 kb, is composed of four exons and three introns, and codes for a 1089 residue polypeptide with a calculated molecular weight of 121, 000. The amino acid sequence has 60% identity with the human and rabbit RGL. The 5′ flanking region of the gene contains a TATA box, c-Myc sites, and a potential cAMP-responsive element. Muscle specific motifs, such as MyoD and MEF-2, were also found. The A-T rich 3′-UTR contained several polyadenylation signals, two associated with poly(A) downstream consensus motifs. ARE elements, which regulate mRNA stability, were dispersed throughout the 3′-UTR. Northern analysis of poly(A) mRNA from various murine tissues indicates a major transcript of 7.5 kb in skeletal muscle and heart. Western analysis demonstrates that RGL protein is present in skeletal and cardiac muscle from mouse, rat, and rabbit but not in L6 myoblasts, L6 myotubes, 3T3 L1 fibroblasts, 3T3 L1 or rat primary adipocytes, confirming that expression of the gene is specific to striated muscle. Analysis of skeletal muscle from rats made diabetic by streptozotocin treatment reveals that the level of RGL protein is the same as in control animals, indicating that expression is not regulated by insulin.

本文言語English
ページ(範囲)135-145
ページ数11
ジャーナルArchives of Biochemistry and Biophysics
388
1
DOI
出版ステータスPublished - 2001 4 1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

フィンガープリント 「Gene structure and expression of the targeting subunit, R<sub>GL</sub>, of the muscle-specific glycogen-associated type 1 protein phosphatase, PP1G」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル