Gene manipulation of chick embryos in vitro, early chick culture, and long survival in transplanted eggs

Jun Tanaka, Hidekiyo Harada, Kodai Ito, Toshihiko Ogura, Harukazu Nakamura

研究成果: Article査読

8 被引用数 (Scopus)

抄録

We introduce a revolutionary gene transfer system in chick: transfect chick embryos at early developmental stage by electroporation in vitro, Early Chick (EC) culture, and transplant to the egg to let the embryo survive until E5.5. Referring to the fate map, we could target the tissues of transfection, or transfect large areas of the embryo. We could get tissue-specific expression of a transgene by tissue-specific promoter. This method is very convenient and rapid, but allows us to get stable expression of the transgene in combination with transposon system.

本文言語English
ページ(範囲)629-634
ページ数6
ジャーナルDevelopment Growth and Differentiation
52
7
DOI
出版ステータスPublished - 2010 9

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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