Gene cloning and biochemical characterization of a catalase from Gluconobacter oxydans

Haruhiko Yamaguchi, Keigo Sugiyama, Miho Hosoya, Seiji Takahashi, Toru Nakayama

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Gluconobacter oxydans has a large number of membrane-bound dehydrogenases linked to the respiratory chain that catalyze incomplete oxidation of a wide range of organic compounds by oxidative fermentation. Because the respiratory chain is a primary site of reactive oxygen species (ROS) production, the bacterium is expected to have a high capacity to detoxify nascent ROS. In the present study, a gene that encodes a catalase of G. oxydans, which might act as a potential scavenger of H2O2, was cloned, and the expression product (termed rGoxCat) was characterized biochemically. rGoxCat is a heme b-containing tetrameric protein (molecular mass, 320kDa) consisting of identical subunits. The recombinant enzyme displayed a strong catalase activity with a kcat of 6.28×104s-1 and a Km for H2O2 of 61mM; however, rGoxCat exhibited no peroxidase activity. These results, along with the phylogenetic position of the enzyme, provide conclusive evidence that rGoxCat is a monofunctional, large-subunit catalase. The enzyme was most stable in the pH range of 4-9, and greater than 60% of the original activity was retained after treatment at pH 3.0 and 40°C for 1h. Moreover, the enzyme exhibited excellent thermostability for a catalase from a mesophilic organism, retaining full activity after incubation for 30min at 70°C. The observed catalytic properties of rGoxCat, as well as its stability in a slightly acidic environment, are consistent with its role in the elimination of nascent H2O2 in a bacterium that produces a large amount of organic acid via oxidative fermentation.

本文言語English
ページ(範囲)522-527
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
111
5
DOI
出版ステータスPublished - 2011 5月

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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