Gelatin-Polyaniline Composite Nanofibers Enhanced Excitation-Contraction Coupling System Maturation in Myotubes

Serge Ostrovidov, Majid Ebrahimi, Hojae Bae, Hung Kim Nguyen, Sahar Salehi, Sang Bok Kim, Akichika Kumatani, Tomokazu Matsue, Xuetao Shi, Ken Nakajima, Shizu Hidema, Makoto Osanai, Ali Khademhosseini

研究成果: Article査読

29 被引用数 (Scopus)

抄録

In this study, composite gelatin-polyaniline (PANI) nanofibers doped with camphorsulfonic acid (CSA) were fabricated by electrospinning and used as substrates to culture C2C12 myoblast cells. We observed enhanced myotube formation on composite gelatin-PANI nanofibers compared to gelatin nanofibers, concomitantly with enhanced myotube maturation. Thus, in myotubes, intracellular organization, colocalization of the dihydropyridine receptor (DHPR) and ryanodine receptor (RyR), expression of genes correlated to the excitation-contraction (E-C) coupling apparatus, calcium transients, and myotube contractibility were increased. Such composite material scaffolds combining topographical and electrically conductive cues may be useful to direct skeletal muscle cell organization and to improve cellular maturation, functionality, and tissue formation.

本文言語English
ページ(範囲)42444-42458
ページ数15
ジャーナルACS Applied Materials and Interfaces
9
49
DOI
出版ステータスPublished - 2017 12 13

ASJC Scopus subject areas

  • 材料科学(全般)

フィンガープリント

「Gelatin-Polyaniline Composite Nanofibers Enhanced Excitation-Contraction Coupling System Maturation in Myotubes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル