Gas chromatographic-mass spectrometric determination of urinary oxoacids using O-(2,3,4,5,6-pentafluorobenzyl)oxime-trimethylsilyl ester derivatization and cation-exchange chromatography

Seon Hwa Lee, Soon Ok Kim, Bong Chul Chung

研究成果: Article査読

17 被引用数 (Scopus)

抄録

We introduced a new combined method to isolate, purify and quantify oxoacids in human urine. Preparation of O-(2,3,4,5,6-pentafluorobenzyl) oximes of oxoacids at pH 2 to 3 was followed by cation-exchange column chromatography for removing the biological interferences. The effluent with water was extracted with ethyl acetate and the oxoacids were quantitatively converted into their trimethylsilyl derivatives for detection by gas chromatography-mass spectrometry. Good quality control data were obtained through precision and accuracy tests. Analytical recoveries (53.5~99.8%) were quantitative for a wide variety of oxoacids. This method was used for the measurement of 18 oxoacids in the urine of healthy volunteers. Copyright (C) 1998 Elsevier Science B.V.

本文言語English
ページ(範囲)1-7
ページ数7
ジャーナルJournal of Chromatography B: Biomedical Applications
719
1-2
DOI
出版ステータスPublished - 1998 11 20
外部発表はい

ASJC Scopus subject areas

  • 化学 (全般)

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