Gambierol Blocks a K⁺ Current Fraction without Affecting Catecholamine Release in Rat Fetal Adrenomedullary Cultured Chromaffin Cells

Gambierol inhibits voltage-gated K⁺ (K_V) channels in various excitable and non-excitable cells. The purpose of this work was to study the effects of gambierol on single rat fetal (F19–F20) adrenomedullary cultured chromaffin cells. These excitable cells have different types of K_V channels and release catecholamines. Perforated whole-cell voltage-clamp recordings revealed that gambierol (100 nM) blocked only a fraction of the total outward K⁺ current and slowed the kinetics of K⁺ current activation. The use of selective channel blockers disclosed that gambierol did not affect calcium-activated K⁺ (K_Ca) and ATP-sensitive K⁺ (K_ATP) channels. The gambierol concentration necessary to inhibit 50% of the K⁺ current-component sensitive to the polyether (IC₅₀) was 5.8 nM. Simultaneous whole-cell current-clamp and single-cell amperometry recordings revealed that gambierol did not modify the membrane potential following 11s depolarizing current-steps, in both quiescent and active cells displaying repetitive firing of action potentials, and it did not increase the number of exocytotic catecholamine release events, with respect to controls. The subsequent addition of apamin and iberiotoxin, which selectively block the K_Ca channels, both depolarized the membrane and enhanced by 2.7 and 3.5-fold the exocytotic event frequency in quiescent and active cells, respectively. These results highlight the important modulatory role played by K_Ca channels in the control of exocytosis from fetal (F19–F20) adrenomedullary chromaffin cells.