Gain- and loss-of-function in chick embryos by electroporation

Harukazu Nakamura, Tatsuya Katahira, Tatsuya Sato, Yuji Watanabe, Jun Ichi Funahashi

研究成果: Review article査読

93 被引用数 (Scopus)

抄録

It remained very difficult to manipulate gene expression in chick embryos until the advent of in ovo electroporation which enabled the induction of both gain-of-function, and recently loss-of-function, of a gene of interest at a specific developmental stage. Gain-of-function by electroporation is so effective that it has become widely adopted in developmental studies in the chick. Recently, it became possible to induce loss-of-function by introducing an siRNA expression vector by electroporation. In this review, the methods of electroporation for gain-of-function and for loss-of-function by siRNA are discussed.

本文言語English
ページ(範囲)1137-1143
ページ数7
ジャーナルMechanisms of Development
121
9
DOI
出版ステータスPublished - 2004 9

ASJC Scopus subject areas

  • 胎生学
  • 発生生物学

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