We have detected both germ-line and somatic p53 mutations in lymphocytes, cell lines and tumor tissues using a functional analysis of p53 tumor suppressor gene based on yeast transcription assay. Through our screening projects of the p53 gene, a number of missense p53 mutations were identified as loss-of-function mutations. This method, previously termed FASAY, is rapid, sensitive, less -expensive and can be automated for screening both somatic and germ-line p53 mutations.
|ジャーナル||Japanese Journal of Cancer and Chemotherapy|
|出版ステータス||Published - 1997 9 24|
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