TY - JOUR
T1 - Functional analysis of the tissue-engineered stomach wall
AU - Hori, Yoshio
AU - Nakamura, Tatsuo
AU - Kimura, Dai
AU - Kaino, Kenji
AU - Kurokawa, Yoshimochi
AU - Satomi, Susumu
AU - Shimizu, Yasuhiko
PY - 2002
Y1 - 2002
N2 - We have established a method for in situ tissue engineering of the stomach in a canine model using an acellular collagen scaffold graft. The current study was conducted to evaluate the functional aspects of the tissueengineered stomach wall. The anterior wall of the stomach in beagle dogs was replaced with a collagen sponge scaffold measuring 4 × 4 cm. At 16 weeks after implantation, the animals were sacrificed and the stomach specimens were evaluated immunohistochemically and physiologically. Regeneration of the proton pump and thin muscle layer, which are essential for mechanical and chemical digestion by the stomach, was observed in the tissueengineered gastric tissue. However, acetylcholine-induced contraction was not observed in the tissue-engineered stomach wall. Although there is still room for improvement, the tissue-engineered stomach wall had a highly organized structure, and it is anticipated that this approach could eventually become an alternative for stomach reconstruction after gastrectomy.
AB - We have established a method for in situ tissue engineering of the stomach in a canine model using an acellular collagen scaffold graft. The current study was conducted to evaluate the functional aspects of the tissueengineered stomach wall. The anterior wall of the stomach in beagle dogs was replaced with a collagen sponge scaffold measuring 4 × 4 cm. At 16 weeks after implantation, the animals were sacrificed and the stomach specimens were evaluated immunohistochemically and physiologically. Regeneration of the proton pump and thin muscle layer, which are essential for mechanical and chemical digestion by the stomach, was observed in the tissueengineered gastric tissue. However, acetylcholine-induced contraction was not observed in the tissue-engineered stomach wall. Although there is still room for improvement, the tissue-engineered stomach wall had a highly organized structure, and it is anticipated that this approach could eventually become an alternative for stomach reconstruction after gastrectomy.
KW - Collagen
KW - Gastrointestinal tract
KW - Reconstruction
KW - Regenerative medicine
KW - Stomach
KW - Tissue engineering
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U2 - 10.1046/j.1525-1594.2002.07006.x
DO - 10.1046/j.1525-1594.2002.07006.x
M3 - Article
C2 - 12296927
AN - SCOPUS:0036906818
VL - 26
SP - 868
EP - 872
JO - Artificial Organs
JF - Artificial Organs
SN - 0160-564X
IS - 10
ER -