Functional analysis of Slac2-a/melanophilin as a linker protein between Rab27A and myosin Va in melanosome transport

Taruho S. Kuroda, Takashi Itoh, Mitsunori Fukuda

研究成果: Review article査読

25 被引用数 (Scopus)

抄録

Slac2-a/melanophilin regulates melanosome transport in mammalian skin melanocytes by linking melanosome-bound Rab27A and an actin-based motor protein, myosin Va. Slac2-a consists of an N-terminal Slp homology domain (SHD), which has been identified as a specific GTP-Rab27-binding domain, a myosin Va-binding domain (MBD) in the middle region, and an actin-binding domain (ABD) at the C-terminus. Mutations in the slac2-a/mlph gene cause the abnormal pigmentation (i.e., perinuclear melanosome aggregation in melanocytes) in human Griscelli syndrome type III and in leaden mice because of the inability to form the tripartite protein complex consisting of Rab27A, Slac2-a, and myosin Va. In this chapter we describe the methods, including in vivo melanosome distribution assay combined with dominant-negative approaches and RNA interference technology, that have been used to analyze the function of Slac2-a in melanosome transport in melanocytes.

本文言語English
論文番号37
ページ(範囲)419-431
ページ数13
ジャーナルMethods in enzymology
403
DOI
出版ステータスPublished - 2005
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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