Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation

Shigeki Shibahara, Miki Yoshizawa, Hiroyuki Suzuki, Kazuhisa Takeda, Kuniaki Meguro, Kazuyasu Endo

研究成果: Article査読

171 被引用数 (Scopus)

抄録

We cloned a cDNA coding for a putative human heme oxygenase isozyme, designated type 2 (HO-2), and analyzed its function by transient expression assays. HeLa cells transfected with either HO-2 cDNAs or a cDNA coding for authentic heme oxygenase (HO-1) expressed the activity of heme oxygenase, although no activity was detected in the mock transfected cells. Using specific anti-HO-1 antibody, we showed that expression of a HO-1 cDNA resulted in the increase in its protein levels, but HO-1 protein was not detectable in the cells expressing HO-2 cDNAs. We thus confirmed the functional identity of HO-1 and HO-2. Then, we analyzed their expression in an erythroid cell line, YN-1-0-A. Treatment with hem in or by heat shock (42°C) led to a remarkable increase in the HO-1 mRNA levels, while HO-2 mRNA expression was not induced at all, suggesting that they are under separate regulation. 1993

本文言語English
ページ(範囲)214-218
ページ数5
ジャーナルJournal of biochemistry
113
2
DOI
出版ステータスPublished - 1993 2

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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