Functional analysis of an endo-1,6-β-D-glucanase gene (neg-1) from Neurospora crassa

Seiji Oyama, Hirokazu Inoue, Youhei Yamagata, Tasuku Nakajima, Keietu Abe

研究成果: Article査読

5 被引用数 (Scopus)

抄録

The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.

本文言語English
ページ(範囲)1773-1775
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
70
7
DOI
出版ステータスPublished - 2006

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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