Functional analysis of an endo-1,6-β-D-glucanase gene (neg-1) from Neurospora crassa

Seiji Oyama; Hirokazu Inoue; Youhei Yamagata; Tasuku Nakajima; Keietu Abe

doi:10.1271/bbb.50626

Functional analysis of an endo-1,6-β-D-glucanase gene (neg-1) from Neurospora crassa

Seiji Oyama, Hirokazu Inoue, Youhei Yamagata, Tasuku Nakajima, Keietu Abe

農学研究科・生物産業創成科学専攻

研究成果: Article › 査読

5 被引用数 (Scopus)

抄録

The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.

本文言語	English
ページ（範囲）	1773-1775
ページ数	3
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	70
号	7
DOI	https://doi.org/10.1271/bbb.50626
出版ステータス	Published - 2006

ASJC Scopus subject areas

バイオテクノロジー
分析化学
生化学
応用微生物学とバイオテクノロジー
分子生物学
有機化学

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10.1271/bbb.50626

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title = "Functional analysis of an endo-1,6-β-D-glucanase gene (neg-1) from Neurospora crassa",

abstract = "The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.",

keywords = "1,6-β-D-glucanase, Fungal cell wall, Fungal β-1,6-glucanase, β-1,6-glucanase disruptant, β-1,6-glucanase gene",

author = "Seiji Oyama and Hirokazu Inoue and Youhei Yamagata and Tasuku Nakajima and Keietu Abe",

note = "Funding Information: This work was supported in part by a Grant-in-Aid (Bio Design Program) from the Ministry of Agriculture, Forestry and Fisheries of Japan.",

year = "2006",

doi = "10.1271/bbb.50626",

language = "English",

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T1 - Functional analysis of an endo-1,6-β-D-glucanase gene (neg-1) from Neurospora crassa

AU - Oyama, Seiji

AU - Inoue, Hirokazu

AU - Yamagata, Youhei

AU - Nakajima, Tasuku

AU - Abe, Keietu

N1 - Funding Information: This work was supported in part by a Grant-in-Aid (Bio Design Program) from the Ministry of Agriculture, Forestry and Fisheries of Japan.

PY - 2006

Y1 - 2006

N2 - The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.

AB - The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.

KW - 1,6-β-D-glucanase

KW - Fungal cell wall

KW - Fungal β-1,6-glucanase

KW - β-1,6-glucanase disruptant

KW - β-1,6-glucanase gene

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

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Functional analysis of an endo-1,6-β-D-glucanase gene (neg-1) from Neurospora crassa

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