抄録
The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.
本文言語 | English |
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ページ(範囲) | 1773-1775 |
ページ数 | 3 |
ジャーナル | Bioscience, Biotechnology and Biochemistry |
巻 | 70 |
号 | 7 |
DOI | |
出版ステータス | Published - 2006 |
ASJC Scopus subject areas
- バイオテクノロジー
- 分析化学
- 生化学
- 応用微生物学とバイオテクノロジー
- 分子生物学
- 有機化学