α1,6-Fucosylation of asparagine-linked oligosaccharides (N-glycans) is ubiquitously observed in eukaryote except plant and fungi. This type of fucosylation is catalyzed by eukaryotic α1,6-fucosyltransferase, and this enzyme is called as FUT8, notably in mammalian. FUT8 transfers fucose moiety from GDP-β-L-fucose to the innermost GlcNAc residue in N-glycan (Fig. 59.1). FUT8 was purified from porcine brain (Uozumi et al. 1996b) and MKN45 cells, a human gastric cancer cell line (Yanagidani et al. 1997). The cDNAs were successfully cloned from porcine and human (Uozumi et al. 1996b; Yanagidani et al. 1997). α1,6-Fucosylation catalyzed by FUT8 and the resulting α1,6-fucose residue is denoted as core fucosylation and core fucose, respectively.
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