Frequent deletions of material from chromosome arm 1p in oligodendroglial tumors revealed by double‐target fluorescence in situ hybridization and microsatellite analysis

Naoya Hashimoto, Daisuke Ichikawa, Yoshiki Arakawa, Kousei Date, Satoshi Ueda, Yoshio Nakagawa, Akira Horii, Yusuke Nakamura, Tatsuo Abe, Johji Inazawa

研究成果: Article査読

40 被引用数 (Scopus)

抄録

We undertook a cytogenetic analysis of 29 human brain tumors using double‐target fluorescence in situ hybridization (FISH) and focusing on chromosome arm 1p. One or more tumor suppressor genes in this arm have been suggested to be important in a variety of neuroectodermal tumors. The series included 9 oligodendrogliomas, 4 mixed gliomas, 10 astrocytomas, 4 glioblastomas, and 2 central neurocytomas. We hybridized pericentromeric (1q12) and subtelomeric (1p36) DNA probes to cell nuclei prepared from paraffin‐embedded tissues and observed a strikingly high incidence of deletion of at least part of 1p in oligodendrogliomas (100%) and mixed gliomas (75%). The results of the FISH analyses were confirmed by demonstration of loss of heterozygosity for a microsatellite polymorphism in 10 of the 29 tumors. As well as supporting the feasibility of FISH for detecting allelic deletions in chromosomes from paraffin‐embedded tumor samples, the alteration of 1p reported here will contribute to an understanding of the molecular genetic events in oligodendroglial tumor development.

本文言語English
ページ(範囲)295-300
ページ数6
ジャーナルGenes, Chromosomes and Cancer
14
4
DOI
出版ステータスPublished - 1995 12

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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