TY - JOUR
T1 - Free radical scavenger edaravone suppresses X-ray-induced apoptosis through p53 inhibition in MOLT-4 cells
AU - Sasano, Nakashi
AU - Enomoto, Atsushi
AU - Hosoi, Yoshio
AU - Katsumura, Yosuke
AU - Matsumoto, Yoshihisa
AU - Shiraishi, Kenshiro
AU - Miyagawa, Kiyoshi
AU - Igaki, Hiroshi
AU - Nakagawa, Keiichi
PY - 2007
Y1 - 2007
N2 - Edaravone, a clinical drug used widely for the treatment of acute cerebral infarction, is reported to scavenge free radicals. In the present study, we investigated the radioprotective effect of edaravone on X-ray-induced apoptosis in MOLT-4 cells. Apoptosis was determined by the dye exclusion test, Annexin V binding assay, cleavage of caspase, and DNA fragmentation. We found that edaravone significantly suppressed the X-ray-induced apoptosis. The amount of intracellular ROS production was determined by the chloromethyl-2′, 7′-dichlorodihydro-fluorescein diacetate system. We found that the intracellular ROS production by X-irradiation was completely suppressed by the addition of edaravone. The accumulation and phosphorylation of p53 and the expression of p21WAF1, a target protein of p53, which were induced by X-irradiation, were also suppressed by adding edaravone. We conclude that the free radical scavenger edaravone suppresses X-ray-induced apoptosis in MOLT-4 cells by inhibiting p53.
AB - Edaravone, a clinical drug used widely for the treatment of acute cerebral infarction, is reported to scavenge free radicals. In the present study, we investigated the radioprotective effect of edaravone on X-ray-induced apoptosis in MOLT-4 cells. Apoptosis was determined by the dye exclusion test, Annexin V binding assay, cleavage of caspase, and DNA fragmentation. We found that edaravone significantly suppressed the X-ray-induced apoptosis. The amount of intracellular ROS production was determined by the chloromethyl-2′, 7′-dichlorodihydro-fluorescein diacetate system. We found that the intracellular ROS production by X-irradiation was completely suppressed by the addition of edaravone. The accumulation and phosphorylation of p53 and the expression of p21WAF1, a target protein of p53, which were induced by X-irradiation, were also suppressed by adding edaravone. We conclude that the free radical scavenger edaravone suppresses X-ray-induced apoptosis in MOLT-4 cells by inhibiting p53.
KW - Edaravone
KW - Free radical scavenger
KW - MOLT-4 cells
KW - Radioprotective effect
KW - X-ray-induced apoptosis
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UR - http://www.scopus.com/inward/citedby.url?scp=36749011255&partnerID=8YFLogxK
U2 - 10.1269/jrr.07061
DO - 10.1269/jrr.07061
M3 - Article
C2 - 17965547
AN - SCOPUS:36749011255
VL - 48
SP - 495
EP - 503
JO - Journal of Radiation Research
JF - Journal of Radiation Research
SN - 0449-3060
IS - 6
ER -