Formation of hydrogen-producing granules and microbial community analysis in a UASB reactor

Yan Yan Ning; Shao Feng Wang; Da Wei Jin; Hideki Harada; Xian Yang Shi

doi:10.1016/j.renene.2012.10.051

Formation of hydrogen-producing granules and microbial community analysis in a UASB reactor

Yan Yan Ning, Shao Feng Wang, Da Wei Jin, Hideki Harada, Xian Yang Shi

未来科学技術共同研究センター

研究成果: Article › 査読

18 被引用数 (Scopus)

抄録

The effect of substrate concentration on continuous H₂ production from a sucrose-rich synthetic wastewater was investigated in this work. The maximum H₂ production rate of 2.89 m³ H₂/m³/d achieved at 7000 mg COD/L while the peak ethanol concentration of 2840 mg/L was observed at 5000 mg COD/L. The H₂-producing granules with ethanol-type fermentation were successfully cultivated in an upflow anaerobic sludge blanket (UASB) reactor. Denaturing gradient gel electrophoresis analysis demonstrates that the substrate concentration had a considerable effect on the microbial community. After the mature H₂-producing granules were formed, the microbial community structure in the UASB reactor tends to stabilize. The dominant bacterium in the mature H₂-producing granules were composed of Janthinobacterium sp. WPCB148, Clostridium sp. HPB-4, Variovorax paradoxus strain SJ100, Variovorax sp. CN3b and Uncultured bacterium clone C2.

本文言語	English
ページ（範囲）	12-17
ページ数	6
ジャーナル	Renewable Energy
巻	53
DOI	https://doi.org/10.1016/j.renene.2012.10.051
出版ステータス	Published - 2013 5月

ASJC Scopus subject areas

再生可能エネルギー、持続可能性、環境

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1016/j.renene.2012.10.051

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@article{c7b527c41480457691f1ddbc516ef9dc,

title = "Formation of hydrogen-producing granules and microbial community analysis in a UASB reactor",

abstract = "The effect of substrate concentration on continuous H2 production from a sucrose-rich synthetic wastewater was investigated in this work. The maximum H2 production rate of 2.89 m3 H2/m3/d achieved at 7000 mg COD/L while the peak ethanol concentration of 2840 mg/L was observed at 5000 mg COD/L. The H2-producing granules with ethanol-type fermentation were successfully cultivated in an upflow anaerobic sludge blanket (UASB) reactor. Denaturing gradient gel electrophoresis analysis demonstrates that the substrate concentration had a considerable effect on the microbial community. After the mature H2-producing granules were formed, the microbial community structure in the UASB reactor tends to stabilize. The dominant bacterium in the mature H2-producing granules were composed of Janthinobacterium sp. WPCB148, Clostridium sp. HPB-4, Variovorax paradoxus strain SJ100, Variovorax sp. CN3b and Uncultured bacterium clone C2.",

keywords = "Granule, Hydrogen production, PCR-DGGE, Upflow anaerobic sludge blanket (UASB) reactor",

author = "Ning, {Yan Yan} and Wang, {Shao Feng} and Jin, {Da Wei} and Hideki Harada and Shi, {Xian Yang}",

note = "Funding Information: The authors wish to thank the Natural Science Key Foundation of the Anhui Higher Education Institutions ( KJ2011A003 ), the Natural Science Foundation of Anhui province ( 1208085ME61 ), the scientific research project of Huainan mining group ( HNKY- JT-JS-2012-3 ), the Natural Science Foundation of China ( 51278001 ), the professional project of Characteristics of Anhui province ( 2011-8 ), and the NSFC–JST Joint Project ( 20610002 ) for the support of this study.",

year = "2013",

month = may,

doi = "10.1016/j.renene.2012.10.051",

language = "English",

volume = "53",

pages = "12--17",

journal = "Renewable Energy",

issn = "0960-1481",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Formation of hydrogen-producing granules and microbial community analysis in a UASB reactor

AU - Ning, Yan Yan

AU - Wang, Shao Feng

AU - Jin, Da Wei

AU - Harada, Hideki

AU - Shi, Xian Yang

N1 - Funding Information: The authors wish to thank the Natural Science Key Foundation of the Anhui Higher Education Institutions ( KJ2011A003 ), the Natural Science Foundation of Anhui province ( 1208085ME61 ), the scientific research project of Huainan mining group ( HNKY- JT-JS-2012-3 ), the Natural Science Foundation of China ( 51278001 ), the professional project of Characteristics of Anhui province ( 2011-8 ), and the NSFC–JST Joint Project ( 20610002 ) for the support of this study.

PY - 2013/5

Y1 - 2013/5

N2 - The effect of substrate concentration on continuous H2 production from a sucrose-rich synthetic wastewater was investigated in this work. The maximum H2 production rate of 2.89 m3 H2/m3/d achieved at 7000 mg COD/L while the peak ethanol concentration of 2840 mg/L was observed at 5000 mg COD/L. The H2-producing granules with ethanol-type fermentation were successfully cultivated in an upflow anaerobic sludge blanket (UASB) reactor. Denaturing gradient gel electrophoresis analysis demonstrates that the substrate concentration had a considerable effect on the microbial community. After the mature H2-producing granules were formed, the microbial community structure in the UASB reactor tends to stabilize. The dominant bacterium in the mature H2-producing granules were composed of Janthinobacterium sp. WPCB148, Clostridium sp. HPB-4, Variovorax paradoxus strain SJ100, Variovorax sp. CN3b and Uncultured bacterium clone C2.

AB - The effect of substrate concentration on continuous H2 production from a sucrose-rich synthetic wastewater was investigated in this work. The maximum H2 production rate of 2.89 m3 H2/m3/d achieved at 7000 mg COD/L while the peak ethanol concentration of 2840 mg/L was observed at 5000 mg COD/L. The H2-producing granules with ethanol-type fermentation were successfully cultivated in an upflow anaerobic sludge blanket (UASB) reactor. Denaturing gradient gel electrophoresis analysis demonstrates that the substrate concentration had a considerable effect on the microbial community. After the mature H2-producing granules were formed, the microbial community structure in the UASB reactor tends to stabilize. The dominant bacterium in the mature H2-producing granules were composed of Janthinobacterium sp. WPCB148, Clostridium sp. HPB-4, Variovorax paradoxus strain SJ100, Variovorax sp. CN3b and Uncultured bacterium clone C2.

KW - Granule

KW - Hydrogen production

KW - PCR-DGGE

KW - Upflow anaerobic sludge blanket (UASB) reactor

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JO - Renewable Energy

JF - Renewable Energy

SN - 0960-1481

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Formation of hydrogen-producing granules and microbial community analysis in a UASB reactor

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ASJC Scopus subject areas

UN SDG

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