Fluorescence lifetime imaging study of a single cell: Stress-induced environmental change and electric field effects onfluorescence

Nobuhiro Ohta, Takakazu Nakabayashi, Issei Nagao, Masataka Kinjo, Yumiko Aoki, Minoru Tanaka

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

A dramatic change occurs in the cellular microenvironment during cell stress, but it has been difficult to follow these changes in vivo. Here, fluorescence lifetime imaging (FLIM) microscopy has been used to examine stress-induced changes in the microenvironment in a single cell. It is observed that the fluorescence lifetime of HeLa cells expressing an enhanced green fluorescent protein (EGFP)-tudor fusion protein changes under stress. The change in the fluorescence lifetime appears to be due to an alteration in the local electric field in the protein matrix surrounding the chromophore of EGFP. In fact, the fluorescence lifetime of the GFP chromophore in a polyvinyl alcohol film is found to decrease in the presence of an electric field, based on the measurements of the field-induced change in the fluorescence decay profile. The results indicate that the rate of the non-radiative process of the chromophore of GFP is enhanced by an applied electric field. The FLIM method allows noninvasive determination of the status of the individual cells.

本文言語English
ホスト出版物のタイトルReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
DOI
出版ステータスPublished - 2009 6 15
外部発表はい
イベントReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications - San Jose, CA, United States
継続期間: 2009 1 262009 1 29

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
7190
ISSN(印刷版)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
国/地域United States
CitySan Jose, CA
Period09/1/2609/1/29

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 原子分子物理学および光学
  • 放射線学、核医学およびイメージング

フィンガープリント

「Fluorescence lifetime imaging study of a single cell: Stress-induced environmental change and electric field effects onfluorescence」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル