Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis

Toshiyuki Ito, Shugo Oshita, Takakazu Nakabayashi, Fan Sun, Masataka Kinjo, Nobuhiro Ohta

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Fluorescence lifetime images of HeLa cells expressing enhanced green fluorescent protein (EGFP) have been measured as apoptosis is induced by tumor necrosis factor-α (TNF-α) in combination with cycloheximide. The fluorescence lifetime of EGFP is found to decrease after the induction of apoptosis, indicating that the change in environment occurs around the chromophore of EGFP with the apoptosis process. The fluorescence lifetime imaging technique can be used to perform in vivo observation of cell death processes. Fluorescence lifetime measurements are useful to examine the induction of the apoptosis process, even when a morphological change of each cell cannot be observed because of a low spatial resolution.

本文言語English
ページ(範囲)763-767
ページ数5
ジャーナルPhotochemical and Photobiological Sciences
8
6
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 物理化学および理論化学

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