Fluorescence lifetime image of a single halobacterium

Hui Ping Wang, Takakazu Nakabayashi, Kazuo Tsujimoto, Seiji Miyauchi, Naoki Kamo, Nobuhiro Ohta

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Fluorescence lifetime imaging (FLIM) has been reported for a single halobacterium loaded with 2′,7′-bis-(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). FLIM of halobacteria exhibits two distinct fluorescence lifetimes, representing different environments from each other in different halobacteria. The observed difference in the fluorescence lifetime appears to be due to the difference in electric field inside a cell. Fluorescence intensity of BCECF in polyvinyl alcohol film is quenched by an external electric field, indicating that the fluorescence lifetime of BCECF is affected by an electric field.

本文言語English
ページ(範囲)441-444
ページ数4
ジャーナルChemical Physics Letters
442
4-6
DOI
出版ステータスPublished - 2007 7 17
外部発表はい

ASJC Scopus subject areas

  • 物理学および天文学(全般)
  • 物理化学および理論化学

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