TY - JOUR
T1 - Fluorescence from abnormally sterile pollen of the japanese apricot
AU - Mori, Shinnosuke
AU - Shimma, Shuichi
AU - Masuko-Suzuki, Hiromi
AU - Watanabe, Masao
AU - Nakanishi, Tetsu
AU - Tsukioka, Junko
AU - Goto, Katsumi
AU - Fukui, Hiroshi
AU - Hirai, Nobuhiro
N1 - Funding Information:
We thank Ryutaro Tao, Hisayo Yamane, and Takashi Akagi (Graduate School of Agriculture, Kyoto University); Yuto Kitamura (Japanese Apricot Research Laboratory, Wakayama Research Center) for valuable discussion on the pollen development; Mizuho Hanaki (Graduate School of Agriculture, Kyoto University) and Katsutoshi Nishino (Graduate School of Biostudies, Kyoto University) for the mass spectral measurements; Tetsuhito Suzuki (Graduate School of Agriculture, Kyoto University) for the diffuse reflectance spectral measurements; and Keiko Okamoto-Furuta and Haruyasu Kohda (Center for Anatomical, Pathological and Forensic Medical Researches, Graduate School of Medicine, Kyoto University) for their technical assistance with electron microscopy. This work was supported in part by MEXT KAKENHI (grant Numbers 16H06470, 16H06464, 16K21727 to MW), JSPS KAKENHI (grant Numbers 17H00821, 18KT0048, 19K22342 to MW) and JSPS Bilateral Programs (grant Number 18032211-000481 to MW).
Publisher Copyright:
© 2021 Japanese Society for Plant Biotechnology.
PY - 2021
Y1 - 2021
N2 - We observed trees of the Japanese apricot, Prunus mume ‘Nanko’ (Rosaceae), bearing two types of flowers: 34% had blue fluorescent pollen under UV irradiation, and 66% had non-fluorescent pollen. The fluorescent pollen grains were abnormally crushed, sterile, and devoid of intine and pollenkitt. The development of microspores within anthers was investigated: in the abnormally developed anthers, tapetal cells were vacuolated at the unicellular microspore stage, and fluorescent pollen was produced. Compounds responsible for the blue fluorescence of pollen were identified as chlorogenic acid and 1-O-feruloyl-β-D-glucose. The anthers with fluorescent pollen contained 6.7-fold higher and 3.8-fold lower amounts of chlorogenic acid and N1,N5,N10-tri-p-coumaroylspermidine, respectively, compared to those with non-fluorescent pollen. The tapetal vacuolization, highly accumulated chlorogenic acid, and deficiency of N1,N5,N10-tri-p-coumaroylspermidine imply that low-temperature stress during the early unicellular microspore stage caused a failure in microsporogenesis. Furthermore, potential effects of the visual difference on the bee behavior were also discussed through the colorimetry. The sterility, likely induced by low-temperature stress, and the preference of honeybees for fluorescence may reduce the pollination efficiency of P. mume.
AB - We observed trees of the Japanese apricot, Prunus mume ‘Nanko’ (Rosaceae), bearing two types of flowers: 34% had blue fluorescent pollen under UV irradiation, and 66% had non-fluorescent pollen. The fluorescent pollen grains were abnormally crushed, sterile, and devoid of intine and pollenkitt. The development of microspores within anthers was investigated: in the abnormally developed anthers, tapetal cells were vacuolated at the unicellular microspore stage, and fluorescent pollen was produced. Compounds responsible for the blue fluorescence of pollen were identified as chlorogenic acid and 1-O-feruloyl-β-D-glucose. The anthers with fluorescent pollen contained 6.7-fold higher and 3.8-fold lower amounts of chlorogenic acid and N1,N5,N10-tri-p-coumaroylspermidine, respectively, compared to those with non-fluorescent pollen. The tapetal vacuolization, highly accumulated chlorogenic acid, and deficiency of N1,N5,N10-tri-p-coumaroylspermidine imply that low-temperature stress during the early unicellular microspore stage caused a failure in microsporogenesis. Furthermore, potential effects of the visual difference on the bee behavior were also discussed through the colorimetry. The sterility, likely induced by low-temperature stress, and the preference of honeybees for fluorescence may reduce the pollination efficiency of P. mume.
KW - Fluorescence
KW - Pollen
KW - Pollination
KW - Prunus mume
KW - Sterility
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U2 - 10.5511/plantbiotechnology.21.0730a
DO - 10.5511/plantbiotechnology.21.0730a
M3 - Article
AN - SCOPUS:85116065906
VL - 38
SP - 355
EP - 366
JO - Plant Biotechnology
JF - Plant Biotechnology
SN - 1342-4580
IS - 3
ER -